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**链cDNA合成试剂盒

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产品名称: **链cDNA合成试剂盒
产品型号: 20 次 50 次 100 次
产品品牌: Biomiga
产品价格: 0.00 元
折扣价格: 0.00 元
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简单介绍

编号: RT0212-01 规格: 20 次 编号: RT0212-02 规格: 50 次 编号: RT0212-03 规格: 100 次


**链cDNA合成试剂盒  的详细介绍

说明 :

Introduction

The First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system. The amount of starting material can vary from 1 pg to 5 μg of total RNA. Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42-55°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because Synthesis System for RT is not significantly inhibited by ribosomal and transfer RNA, it may be used to synthesize first-strand cDNA from a total RNA preparation.
cDNA synthesis is performed in the first step using either total RNA or poly(A)+-selected RNA primed with oligo(dT), random primers, or a gene-specific primer. In the second step, PCR is performed in a separate tube using primers specific for the gene of interest.
 

Storage and Stability

Store at -20°C，The First-Strand Synthesis System for RT-PCR is stable for 1 year when stored at -20°C.
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